Journal: Cell Reports Methods

Article Title: Global analysis of protein arginine methylation

doi: 10.1016/j.crmeth.2021.100016

Figure Lengend Snippet:

Article Snippet: SW1353 , CLS , Cat # 300440.

Techniques: Virus, Recombinant, Extraction, Reverse Transcription, Single Cell, RNA sequencing, Real-time Polymerase Chain Reaction, Plasmid Preparation, Software

Journal: Nutrients

Article Title: Heat-Killed Lactobacillus delbrueckii subsp. lactis 557 Extracts Protect Chondrocytes from Osteoarthritis Damage by Reducing Inflammation: An In Vitro Study

doi: 10.3390/nu16244417

Figure Lengend Snippet: Effects of LDL557 extracts (LDL557) on SW1353 cell viability and monosodium iodoacetate (MIA, M)-induced cell death. The SW1353 cells were incubated for 24 h in the absence or presence of LDL557 extracts in the range of 3–200 μg/mL ( A ), or the cells were treated with 10 μg/mL LDL557 extracts for 24 h, followed by incubation with or without 5 μM MIA for an additional 24 h. Then, cell viability was determined via crystal violet staining ( B ). Data are presented as a percentage of the control group, accompanied by the mean ± standard deviation (S.D.) from three experiments. Statistically significant differences are indicated by * ( p < 0.05 compared with the untreated control group) and # ( p < 0.05 compared with the MIA-treated group). C = control untreated cells; M = MIA-treated cells; LDL557 = cells treated only with LDL557 extracts; M+LDL557 = cells treated with LDL557 extracts followed by treatment with MIA.

Article Snippet: The SW1353 chondrosarcoma cell line, originally obtained from a human cell line, was procured from the Bioresource Collection and Research Center (BCRC) at the Food Industry Research and Development Institute in Hsinchu, Taiwan.

Techniques: Incubation, Staining, Control, Standard Deviation

Journal: Nutrients

Article Title: Heat-Killed Lactobacillus delbrueckii subsp. lactis 557 Extracts Protect Chondrocytes from Osteoarthritis Damage by Reducing Inflammation: An In Vitro Study

doi: 10.3390/nu16244417

Figure Lengend Snippet: Oxidative stress of SW1353 cells treated with LDL557 extracts or MIA. The details of the treatment with LDL557 extracts and MIA are described in , and flow cytometry was conducted to analyze the levels of ROS ( A ), H 2 O 2 ( B ), and NO ( C ) using DCFH-DA, MitoPY1, and DAF-FM DA staining, respectively. The untreated control is depicted by the color-filled area (yellow, blue, or green), while the treated groups are represented by the thick black lines. Data are presented as a percentage of the control group, accompanied by the mean ± standard deviation (S.D.) from three experiments. Statistically significant differences are indicated by * ( p < 0.05 compared with the untreated control group). C = control untreated cells; M = MIA-treated cells; LDL557 = cells treated only with LDL557 extracts; M+LDL557 = cells treated with LDL557 extracts followed by treatment with MIA.

Article Snippet: The SW1353 chondrosarcoma cell line, originally obtained from a human cell line, was procured from the Bioresource Collection and Research Center (BCRC) at the Food Industry Research and Development Institute in Hsinchu, Taiwan.

Techniques: Flow Cytometry, Staining, Control, Standard Deviation

Journal: Nutrients

Article Title: Heat-Killed Lactobacillus delbrueckii subsp. lactis 557 Extracts Protect Chondrocytes from Osteoarthritis Damage by Reducing Inflammation: An In Vitro Study

doi: 10.3390/nu16244417

Figure Lengend Snippet: Effects of LDL557 extracts or MIA on inflammatory and anti-inflammatory cytokines’ mRNA expression in SW1353 cells. The details of the treatment with LDL557 extracts and MIA are described in . The mRNA expression levels of IL-1β ( A ), IL-6 ( B ), TNF-α ( C ), and IL-10 ( D ) were analyzed via RT-qPCR with β-actin used as the internal control. Data are presented as a fold change of the control group, accompanied by the mean ± standard deviation (S.D.) from three experiments. Statistically significant differences are indicated by * ( p < 0.05 compared with the untreated control group) and # ( p < 0.05 compared with the MIA-treated group). C = control untreated cells; M = MIA-treated cells; LDL557 = cells treated only with LDL557 extracts; M+LDL557 = cells treated with LDL557 extracts followed by treatment with MIA.

Article Snippet: The SW1353 chondrosarcoma cell line, originally obtained from a human cell line, was procured from the Bioresource Collection and Research Center (BCRC) at the Food Industry Research and Development Institute in Hsinchu, Taiwan.

Techniques: Expressing, Quantitative RT-PCR, Control, Standard Deviation

Journal: Nutrients

Article Title: Heat-Killed Lactobacillus delbrueckii subsp. lactis 557 Extracts Protect Chondrocytes from Osteoarthritis Damage by Reducing Inflammation: An In Vitro Study

doi: 10.3390/nu16244417

Figure Lengend Snippet: Effects of LDL557 extracts or MIA on COX-2 and mPGES-1 mRNA expression in SW1353 cells. The details of the treatment with LDL557 extracts and MIA are described in . The mRNA expressions of COX-2 ( A ) and mPGES-1 ( B ) were measured with β-actin used as the internal control. Data are presented as a fold change of the control group, accompanied by the mean ± standard deviation (S.D.) from three experiments. Statistically significant differences are indicated by * ( p < 0.05 compared with the untreated control group). C = control untreated cells; M = MIA-treated cells; LDL557 = cells treated only with LDL557 extracts; M+LDL557 = cells treated with LDL557 extracts followed by treatment with MIA.

Article Snippet: The SW1353 chondrosarcoma cell line, originally obtained from a human cell line, was procured from the Bioresource Collection and Research Center (BCRC) at the Food Industry Research and Development Institute in Hsinchu, Taiwan.

Techniques: Expressing, Control, Standard Deviation

Journal: Arthritis Research & Therapy

Article Title: Chondrocytes, synoviocytes and dermal fibroblasts all express PH-20, a hyaluronidase active at neutral pH

doi: 10.1186/ar1730

Figure Lengend Snippet: Northern blot analysis of mRNA from various connective tissue cell lines. (a) A radiolabelled PH-20 cDNA probe was hybridized to a northern blot containing 25 μg/lane of total RNA from (1) testis (positive control); (2) liver (negative control); (3) synoviocytes; (4) chondrocytes; (5) chondrocytes stimulated with 5 ng/ml of IL-1; (6) chondrocytes stimulated with 10 ng/ml of IL-1; (7) fibroblasts; (8) a chondrosarcoma cell line. (b) For loading control, the same blot was stripped and hybridized with a β-actin probe. (c) Column box–whisker plot showing the relative increase of the PH-20 2.4-kilobase transcript in chondrocytes stimulated with IL-1 at two concentrations ( n = 5; * P = 0.0004 by paired t -test).

Article Snippet: Human chondrosarcoma cells (SW 1353) were purchased from the American Tissue Culture Collection (LGC Promochem, Teddington, UK).

Techniques: Northern Blot, Positive Control, Negative Control, Control, Whisker Assay

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: The infiltration profile of different immune cells in TME for men and women with osteosarcoma. The analysis was performed using (A) CIBERSORT, (B) MCP‐counter, (C) ssGSEA, and (D) Xcell computational method

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques:

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Consistent clustering diagram of immune cells in different clusters. A heatmap showing the distribution of immune genes (A), and four subgroups of CDF (B). (C) A heatmap showing the infiltration level of immune cells in the four osteosarcoma subgroups. The clusters were generated based on TP, ISs, and SSs

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Generated

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Box plot displaying the expression pattern of immune‐related genes between osteosarcoma subgroups. (A and B) ISs and SSs for the four osteosarcoma clusters. Compared with the other three clusters, cluster 4 had a higher immune score. (C–F) The expression levels of multiple immune genes among the four clusters. *, p < 0.05. **, p < 0.01. ***, p < 0.001. ****, p < 0.0001

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Expressing

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Distribution of the main pathological osteosarcoma subtypes in the four osteosarcoma clusters. (A–D) The major pathological subtypes including Leiomyosarcoma (LMS), Myxofibrosarcoma (MF), undifferentiated pleomorphic sarcoma (MFH), and Undifferentiated Pleomorphic Sarcoma (UPS) in the four clusters. (E and F) The survival rate of patients in different subtypes

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques:

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Validation of NPTX2 function in osteosarcoma. (A–C) Association of NPTX2 mRNA with CD8A, IFNG, and GZMB expression in osteosarcoma tissues ( n = 35). (D) The knockdown efficacy of NPTX2 in HOS and SW1353 cells as determined by qPCR. (E and F) Cell proliferation was measured with the CCK8 assays in HOS and SW1353 cells transfected with siRNAs or NC. (G and H) Apoptosis of HOS cells and SW1353 cells after transfection with siRNAs or NC was detected by flow cytometric assay. (I and J) The expression of CCL4, CCL5, CXCL9, CXCL10, and CXCL13 as measured with qPCR in HOS‐NPTX2‐NC and HOS‐NPTX2‐siRNAs cells, or SW1353‐NPTX2‐NC and SW1353‐NPTX2‐siRNAs cells. (K and L) The expression of CCL4, and CXCL13 as quantified with ELISA assay. (M and N) Association of NPTX2 mRNA with CCL4 expression in the TCGA datasets and in osteosarcoma tissues from our patients ( n = 35). (O and P) Association of CCL4 mRNA with CD8A expression in the TCGA datasets and osteosarcoma tissues from our patients ( n = 35)

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Biomarker Discovery, Expressing, Knockdown, Transfection, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Hub genes for the osteosarcoma prognostic model. (A) Multivariate cox regression analysis of hub genes. (B) Kaplan–Meier curves showing the OS of patients with high and low prognostic scores. (C) The time‐dependent ROC curves displaying the prognostic prediction of the hub genes model

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques:

Journal: Cancer Medicine

Article Title: A comprehensive analysis of immune infiltration in the tumor microenvironment of osteosarcoma

doi: 10.1002/cam4.4117

Figure Lengend Snippet: Relationship between clinical features and prognosis of osteosarcoma. (A) Multivariate cox regression analysis results indicating the relationship between metastasis, risk score, and OS. (B) The ROC showing the prognostic accuracy of the 11‐gene model across several cancer and disease factors (gender, across age, in metastatic, and non‐metastatic tumors as well as depth, length, and width). (C) The predictive accuracy of the 11‐gene risk score model in predicting immunotherapy response. The low‐risk group had better OS than the high‐risk group ( p < 0.0001)

Article Snippet: Human osteosarcoma cancer cell lines (HOS, SW1353) were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: